IL-12/IL-23 p40, PerCP-eFluor™ 710, clone: eBioHP40 (HP40, HP-40), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-7235-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The eBioHP40 antibody reacts with the p40 subunit of human IL-12 p70, and human IL-23. IL-12 is a 70kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.The eBioHP40 antibody has slightly lower background staining than C8.6 and recognizes a different epitope.Applications Reported: This eBioHP40 (HP40, HP-40) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBioHP40 (HP40, HP-40) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|IL12B, Il12p40, interleukin 12B, RP23-388G23.1, Il-12b, Il-12p40, p40, IL-12 p40, IL-23 subunit p40, IL12P40|
|HP-40), eBioHP40 (HP40|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.