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IL-10, Functional Grade, clone: JES5-2A5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-7102-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15288757

  • £62.18 / 50µg


Estimated Shipment
05-07-2018
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Description

Description

Description: The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody is a neutralizing antibody. Mouse IL-10 is an 18kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.Applications Reported: The JES5-2A5 antibody has been reported for use in ELISA, ELISPOT, neutralization of IL-10 bioactivity, and intracellular staining for flow cytometric analysis.Applications Tested: The Functional Grade Purified JES5-2A5 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-10 bioactivity. The JES5-2A5 antibody at 0.004 μg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-10 in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. The biotinylated JES5-2A5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse interleukin-10 (IL-10) in combination with the purified JES5-16E3 (14-7101) antibody for capture and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

IL-10
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P18893
Interleukin-10, CSIF, IL-10, IL10A, TGIF
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Cytokine Neutralization
JES5-2A5
Functional Grade
Il10
Purified
Il10
IgG1, kappa
50μg
Store at 2-8°C.
Primary
16153
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