missing translation for 'onlineSavingsMsg'
Learn More

IL-10, Functional Grade, clone: JES5-16E3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-7101-85

Code : Z2

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15278757

  • £174.98 / 500µg


Estimated Shipment
26-02-2018
Add to basket

Disclaimers:

For Research Use Only.


Description

Description

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ∽18kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.Applications Tested: The Functional Grade purified JES5-16E3 antibody has been tested for ELISPOT capture. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 μg/mL. The JES5-16E3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-10 (IL-10) in combination with the biotinylated JES5-2A5 (13-7102) antibody for detection and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

IL-10
JES5-16E3
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
ELISPOT Capture Antibody
1mg/mL
aqueous buffer, no sodium azide
Interleukin-10, IL10
IgG2b
RUO
Primary
Murine
Videos