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IL-10, Alexa Fluor 700, clone: JES5-16E3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-7101-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15392150

  • £89.00 / 25µg

Estimated Shipment date
from Supplier 25-04-2018
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Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ∽18kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.Applications Reported: This JES5-16E3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This JES5-16E3 antibody has been tested by intracellular staining and flow cytometric analysis on stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.


Alexa Fluor 700
Store at 2-8°C. Do not freeze. Light-sensitive material.
Flow Cytometry (Intracellular Staining)
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
IgG2b, kappa

For Research Use Only.