IL-10, Alexa Fluor 488, clone: JES5-16E3, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 53-7101-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ∽18kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.Applications Tested: The Alexa Fluor® 488 conjugated JES5-16E3 antibody has been tested by intracellular immunofluorescent staining with flow cytometric analysis and can be used at less than or equal to 0.50μg per million cells in 100 ul volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Alexa Fluor 488|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow Cytometry (Intracellular Staining)|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
For Research Use Only.