IL-10 Monoclonal Antibody (JES5-16E3), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15322070	Affymetrix eBioscience 53-7101-80	25 μg
15332070	Affymetrix eBioscience 53-7101-82	100 μg

Description

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ∽18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response. Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity. Applications Tested: The Alexa Fluor™ 488 conjugated JES5-16E3 antibody has been tested by intracellular immunofluorescent staining with flow cytometric analysis and can be used at less than or equal to 0.50 μg per million cells in 100 μL volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

IL-10 Monoclonal antibody specifically detects IL-10 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: IL-10
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
• Gene Symbols: IL10
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: IL10

• Clone: JES5-16E3
• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 488
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P18893
• Gene ID (Entrez): 16153
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.