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IL-1 beta (Pro-form), eFluor 450, clone: NJTEN3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-7114-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15519806

  • £87.34 / 25µg


EU Stock 2
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Description

Description

Description: This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, which is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. IL-1 beta signals via the IL-1RI, which is shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.The NJTEN3 antibody recognizes only the pro-form of mouse IL-1 beta and does not see the active (cleaved) form.Applications Reported: This NJTEN3 antibody has been reported for use intracellular staining followed by flow cytometric analysis.Applications Tested: This NJTEN3 antibody has been tested by intracellular staining of stimulated mouse thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-1 beta
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P10749
Interleukin-1 beta, IL-1F2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
NJTEN3
eFluor 450
Il1b
Conjugated
Il1b
IgG1, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16176
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