Rat IgM, PE-eFluor 610, clone: eBRM, Isotype Control, eBioscience™
Rat Isotype Control
Brand: Affymetrix eBioscience 61-4341-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal rat IgM K is useful as an isotype control immunoglobulin.Applications Reported: Rat IgM Isotype Control PE-eFluor® 610 has been reported for use in flow cytometric analysis.Applications Tested: Rat IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|