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Rat IgM, PE-eFluor 610, clone: eBRM, Isotype Control, eBioscience™

Rat Isotype Control

Brand:  Affymetrix eBioscience 61-4341-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15570497

  • £55.92 / 25µg


Estimated Shipment date
from Supplier 04-06-2018
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Description

Description

Description: The monoclonal rat IgM K is useful as an isotype control immunoglobulin.Applications Reported: Rat IgM Isotype Control PE-eFluor® 610 has been reported for use in flow cytometric analysis.Applications Tested: Rat IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgM
Control
PE-eFluor 610
Conjugated
Rat
Affinity chromatography
RUO
Isotype Control
Flow Cytometry
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
IgM, IgM Mu
IgM, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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