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IgM Monoclonal Antibody (II/41), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5790-80
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM. Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis. Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
IgM Monoclonal antibody specifically detects IgM in Mouse samples. It is validated for Flow Cytometry.Specifications
IgM | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P01872 | |
Ighm | |
Affinity chromatography | |
RUO | |
16019 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
II/41 | |
PerCP-eFluor 710 | |
Ighm | |
AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH | |
Rat | |
25 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.