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Mouse IgM, clone: II/41, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5790-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15217477

  • £62.79 / 100µg


Estimated Shipment
26-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: The II/41 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA.Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgM
II/41
Unconjugated
Purified
Rat
100μg
Store at 2-8°C.
Monoclonal
Immunohistochemistry (Frozen)
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Immunoglobulin M
IgG2a
RUO
Primary
Murine
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