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Mouse IgM, Biotin, clone: II/41, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5790-85

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15209859

  • £99.54 / 500Āµg


Estimated Shipment date
from Supplier 02-07-2018
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Description

Description

Description: The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: The II/41 antibody has been reported for use in flow cytometric analysis, immunohistochemistry, and immunocytochemistry.Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgM
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Conjugated
Ighm
IgG2a, kappa
500μg
Store at 2-8°C. Do not freeze.
Primary
16019
Flow CytometryĆ¾ImmunocytochemistryĆ¾Immunohistochemistry
II/41
Biotin
Ighm
Immunoglobulin M
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
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