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Human IgM, Biotin, clone: SA-DA4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-9998-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15300160

  • £68.27 / 100Āµg


EU Stock 1
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Description

Description

Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.Applications Reported: The SA-DA4 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining.Applications Tested: The SA-DA4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgM
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01871
IGHM
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze.
Primary
3507
Flow CytometryĆ¾Immunohistochemistry
SA-DA4
Biotin
IGHM
Conjugated
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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