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Human IgM, APC, clone: SA-DA4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-9998-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15578296

  • £177.54 / 100 tests


EU Stock 2
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For Research Use Only.


Description

Description

Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.Applications Tested: This SA-DA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgM
SA-DA4
APC
Conjugated
IgG1
RUO
Primary
Human
Immunohistochemistry (Frozen)
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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