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Rat anti-Mouse IgG2b, FITC, clone: m2b-25G4, Secondary Antibody, eBioscience™

Rat Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 11-4220-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15586526

  • £102.91 / 100µg


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Description

Description

Description: The monoclonal antibody m2b-25G4 recognizes Mouse IgG2b antibodies and can be used as a second step reagent in flow cytometry and microscopy. This m2b-25G4 antibody does not recognize other mouse isotypes nor does it crossreact to rat IgG2b or any rat isotype antibodies.Applications Reported: This m2b-25G4 antibody has been reported for use in flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This m2b-25G4 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG2b primary antibody. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This m2b-25G4 antibody has also been tested by immunocytochemistry of fixed and permeabilized cells stained with a mouse IgG2b primary antibody. This can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgG2b
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Rat
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
Flow CytometryþImmunocytochemistryþImmunohistochemistryþMicroscopy
m2b-25G4
FITC
Conjugated
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Secondary
Murine
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For Research Use Only.