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Mouse anti-Rat IgG2b, eFluor 660, Clone: R2B-7C3, Secondary Antibody, eBiocience™

Mouse Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 50-4815-80

Code : NEW

Additional Details : Weight : 0.09500kg

Product Code. 15579956

  • £71.50 / 25µg

Estimated Shipment

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Description: The monoclonal antibody R2B-7C3 recognizes Rat IgG2b antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2b or any mouse isotype antibodies.Applications Reported: This R2B-7C3 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This R2B-7C3 antibody has been tested by immunohistochemistry on frozen mouse spleen stained first with a primary antibody of the rat IgG2b isotype and can be used at less than equal to 20 μg/mL. This R2B-7C3 antibody has also been tested by flow cytometric analysis of cells stained with a primary antibody of the rat IgG2b isotype and can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.


Rat IgG2b
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Affinity chromatography
Secondary Antibody
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Microscopy
eFluor 660
IgG2b, kappa, kappa
Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.