Mouse anti-Rat IgG2b Secondary Antibody, eFluor™ 570, eBioscience™, Invitrogen™

Mouse Monoclonal Secondary Antibody

Product Code	Brand	Quantity
15528946	Affymetrix eBioscience 41-4815-80	25 μg
15538946	Affymetrix eBioscience 41-4815-82	100 μg

Description

Description: The monoclonal antibody R2B-7C3 recognizes Rat IgG2b antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2b or any mouse isotype antibodies. Applications Reported: This R2B-7C3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This R2B-7C3 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded sections that have been stained first with a rat IgG2b anti-human antibody. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm

Rat IgG2b Monoclonal antibody specifically detects Rat IgG2b in Rat samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).

Specifications

• Antigen: Rat IgG2b
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: R2B-7C3
• Applications: Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Conjugate: eFluor 570
• Host Species: Mouse
• Target Species: Rat
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography

For Research Use Only.