Mouse IgG2b kappa, PE-Cyanine5, clone: eBMG2b, Isotype Control, eBioscience™

Mouse Isotype Control

Brand:  Affymetrix eBioscience 15-4732-42

Product Code. 15587756

Description

Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.Applications Reported: This mouse IgG2b isotype control has been reported for use in flow cytometric analysis.Applications Tested: Mouse IgG2b K Isotype Control PE-Cy5 has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Mouse IgG2b kappa
• Classification: Control
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: IgG2, IgG2b, IgG2bk
• Isotype: IgG2b, kappa, kappa
• Quantity: 100 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Control, Flow Cytometry
• Clone: eBMG2b
• Conjugate: PE-Cyanine5
• Format: Liquid
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Isotype Control
• Target Species: Not Applicable

For Research Use Only.