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Mouse IgG2b kappa, PE-Cyanine5, clone: eBMG2b, Isotype Control, eBioscience™

Mouse Isotype Control

Brand:  Affymetrix eBioscience 15-4732-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15577756

  • £61.00 / 25 tests


Estimated Shipment
11-06-2021
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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.Applications Reported: This mouse IgG2b isotype control has been reported for use in flow cytometric analysis.Applications Tested: Mouse IgG2b K Isotype Control PE-Cy5 has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Mouse IgG2b kappa
Control
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
IgG2, IgG2b, IgG2bk
IgG2b, kappa, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry
eBMG2b
PE-Cyanine5
Liquid
Murine
Affinity chromatography
RUO
Isotype Control
Not Applicable
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For Research Use Only.