Rat anti-Mouse IgG2a, FITC, clone: m2a-15F8, Secondary Antibody, eBioscience™

Rat Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 11-4210-80

Product Code. 15556526

Description

Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies.Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining and immunocytochemistry.Applications Tested: This m2a-15F8 antibody has been tested by immunocytochemistry on fixed MDCK cells at less than or equal to 20 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: IgG2a
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Secondary Antibody
• Monoclonal or Polyclonal: Monoclonal

• Applications: ImmunocytochemistryþImmunohistochemistry
• Clone: m2a-15F8
• Conjugate: FITC
• Format: Conjugated
• Isotype: IgG1, kappa
• Quantity: 25μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Secondary
• Target Species: Murine

For Research Use Only.