Rat anti-Mouse IgG2a Secondary Antibody, eFluor™ 570, eBioscience™, Invitrogen™

Rat Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 41-4210-82

Product Code. 15391060

Description

Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies. Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. Applications Tested: This m2a-15F8 antibody has been tested immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG2a isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm

Mouse IgG2a Monoclonal antibody specifically detects Mouse IgG2a in Mouse samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).

Specifications

• Antigen: Mouse IgG2a
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: m2a-15F8
• Conjugate: eFluor 570
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG1 κ

For Research Use Only.