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Rat anti-Mouse IgG2a, eFluor 570, clone: m2a-15F8, Secondary Antibody, eBioscience™

Rat Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 41-4210-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15391060

  • £174.38 / 100µg


Estimated Shipment
31-07-2018
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Description

Description

Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies.Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry.Applications Tested: This m2a-15F8 antibody has been tested immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG2a isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

IgG2a
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Rat
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
ImmunocytochemistryþImmunohistochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)
m2a-15F8
eFluor 570
Conjugated
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Secondary
Murine
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