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Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Secondary Antibody

£48.28 - £139.00

Specifications

Antigen Rat IgG2a
Clone r2a-21B2
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Product Code Brand Quantity Price Quantity & Availability  
15509966
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Affymetrix eBioscience
50-4817-82
100 μg
£139.00
100µg
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15599956
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Affymetrix eBioscience
50-4817-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This r2a-21B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This r2a-21B2 antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells stained with a rat IgG2a primary antibody. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2a Monoclonal antibody specifically detects Rat IgG2a in Rat samples. It is validated for Flow Cytometry.
Specifications

Specifications

Rat IgG2a
0.2 mg/mL
Monoclonal
Liquid
RUO
IgG1 κ
Affinity chromatography
r2a-21B2
Flow Cytometry
eFluor 660
Mouse
Rat
Secondary
4° C, store in dark, DO NOT FREEZE!
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