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Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 660, eBioscience™, Invitrogen™
Mouse Monoclonal Secondary Antibody
Brand: Affymetrix eBioscience 50-4817-82
Code : NEW
Additional Details : Weight : 0.09500kg
Description
Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This r2a-21B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This r2a-21B2 antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells stained with a rat IgG2a primary antibody. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
Rat IgG2a Monoclonal antibody specifically detects Rat IgG2a in Rat samples. It is validated for Flow Cytometry.Specifications
| Rat IgG2a | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Affinity chromatography | |
| RUO | |
| Rat | |
| Liquid |
| Flow Cytometry | |
| r2a-21B2 | |
| eFluor 660 | |
| Mouse | |
| 100 μg | |
| Secondary | |
| 4° C, store in dark, DO NOT FREEZE! | |
| IgG1 κ |
For Research Use Only.