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Mouse anti-Rat IgG2a, eFluor 615, Clone: r2a-21B2, Secondary Antibody, eBiocience™

Mouse Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 42-4817-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341110

  • £51.85 / 25µg


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Description

Description

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies.Applications Reported: This R2a-21B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry and immunohistochemistry using a rat primary antibody at 10μg/mL. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Rat IgG2a
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Murine
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
r2a-21B2
eFluor 615
Liquid
IgG1, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Secondary
Rat
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For Research Use Only.