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Mouse anti-Rat IgG2a, eFluor 570, clone: r2a-21B2, Secondary Antibody, eBioscience™

Mouse Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 41-4817-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15558946

  • £109.08 / 100µg


EU Stock 2
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Description

Description

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies.Applications Reported: This R2a-21B2 antibody has been reported for use in immunocytochemistry, and immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections.Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry on fixed and permeabilized cells that have been stained first with a rat IgG2a anti-mouse antibody. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

IgG2a
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Murine
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
ImmunocytochemistryþImmunohistochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)
r2a-21B2
eFluor 570
Conjugated
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Secondary
Rat
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