Learn More
Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™, Invitrogen™
Rat Isotype Control
Brand: Affymetrix eBioscience 61-4321-80
Code : NEW
Additional Details : Weight : 0.09500kg
Description
Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody. PE-eFluor™ 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red™). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.
Rat IgG2a kappa Control antibody specifically detects Rat IgG2a kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.Specifications
Rat IgG2a kappa | |
eBR2a | |
PE-eFluor 610 | |
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2 | |
Affinity chromatography | |
RUO | |
Not Applicable | |
Liquid |
Control, Flow Cytometry | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Rat | |
25 μg | |
Isotype Control (Primary) | |
4° C, store in dark, DO NOT FREEZE! | |
IgG2a κ |
For Research Use Only.