Rat IgG2a kappa, PE-eFluor 610, clone: eBR2a, Isotype Control, eBioscience™
Rat Isotype Control
Brand: Affymetrix eBioscience 61-4321-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|