Rat IgG2a kappa, PE-Cyanine5, clone: eBR2a, Isotype Control, eBioscience™
Rat Isotype Control
Brand: Affymetrix eBioscience 15-4321-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA.Applications Tested: Rat IgG2a Isotype Contrl is offered in 2 formats: - μg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by staining and flow cytometric analysis. This can be used at test size: 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|IgG2, IgG2a, IgG2k|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.