Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™, Invitrogen™

Rat Isotype Control

Product Code	Brand	Quantity
15248617	Affymetrix eBioscience 16-4321-81	50 μg
15258617	Affymetrix eBioscience 16-4321-82	100 μg
15268617	Affymetrix eBioscience 16-4321-85	500 μg

Description

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA. Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and can be used at the same concentration as the experimental antibody. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.

Specifications

• Antigen: Rat IgG2a kappa
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Host Species: Rat
• Target Species: Not Applicable
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C

• Clone: eBR2a
• Applications: Control, Flow Cytometry, Functional Assay
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography

For Research Use Only.