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Mouse IgG2a kappa, APC, clone: eBM2a, Isotype Control, eBioscience™

Mouse Isotype Control

Brand:  Affymetrix eBioscience 17-4724-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15538096

  • £60.00 / 25 tests


In stock, ready to ship: 1
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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis.Applications Tested: The APC conjugated mouse IgG2a isotype control is offered in 2 formats: - μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at less than or equal to 1 μg/million cells (100 μL blood). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at test size: 5 μL (1 μg)/test per million cells (100 μL blood).Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Mouse IgG2a kappa
Control
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
IgG2, IgG2a, IgG2ak
IgG2a, kappa, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Monoclonal
Control, Flow Cytometry
eBM2a
APC
Liquid
Mouse
Affinity chromatography
RUO
Isotype Control
Not Applicable
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