Rat anti-Mouse IgG1 Secondary Antibody, PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 25-4015-82

Product Code. 12759716

Description

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Mouse IgG1 Monoclonal antibody specifically detects Mouse IgG1 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Mouse IgG1
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Form: Liquid

• Applications: Flow Cytometry
• Clone: M1-14D12
• Conjugate: PE-Cyanine7
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG

For Research Use Only.