Rat anti-Mouse IgG1, FITC, clone: M1-14D12, Secondary Antibody, eBioscience™
Rat Monoclonal Secondary Antibody
Brand: Affymetrix eBioscience 11-4015-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, and immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections.Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cell after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Flow CytometryþImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.