Rat anti-Mouse IgG1, eFluor 615, Clone: M1-14D12, Secondary Antibody, eBiocience™
Rat Monoclonal Secondary Antibody
Brand: Affymetrix eBioscience 42-4015-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining and immunocytochemistry.Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.