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Rat anti-Mouse IgG1, eFluor 615, Clone: M1-14D12, Secondary Antibody, eBiocience™

Rat Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 42-4015-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341100

  • £139.91 / 100µg


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Description

Description

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining and immunocytochemistry.Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Mouse IgG1
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Rat
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
M1-14D12
eFluor 615
Liquid
IgG
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Secondary
Murine
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For Research Use Only.