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Rat anti-Mouse IgG1, eFluor 450, clone: M1-14D12, Secondary Antibody, eBioscience™

Rat Monoclonal Secondary Antibody

Brand:  Affymetrix eBioscience 48-4015-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15549746

  • £40.55 / 25µg


EU Stock 3
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Description

Description

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of staining with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgG1
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Rat
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
Flow Cytometry
M1-14D12
eFluor 450
Conjugated
IgG
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Secondary
Murine
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