Mouse anti-Rat IgG1, FITC, clone: R1-12D10, Secondary Antibody, eBioscience™
Mouse Monoclonal Secondary Antibody
Brand: Affymetrix eBioscience 11-4812-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG1.Applications Reported: This R1-12D10 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.Applications Tested: This R1-12D10 antibody has been tested by flow cytometric analysis of cells stained with a rat IgG1 primary antibody and can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. This R1-12D10 antibody has also been tested by immunohistochemistry of human tissue stained with a rat IgG1 primary antibody and can be used at less than or equal to 10 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.