Rat IgG1 kappa, PE-eFluor 610, clone: eBRG1, Isotype Control, eBioscience™

Rat Isotype Control

Brand:  Affymetrix eBioscience 61-4301-82

Product Code. 15520497

Description

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This eBRG1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBRG1 antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: IgG1, K
• Classification: Control
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: IgG1, IgG1k
• Isotype: IgG1, kappa
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Clone: eBRG1
• Conjugate: PE-eFluor 610
• Format: Conjugated
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Isotype Control

For Research Use Only.