Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Isotype Control

Brand:  Affymetrix eBioscience 25-4301-81

Product Code. 15231039

Description

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG1 K Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG1 kappa Control antibody specifically detects Rat IgG1 kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.

Specifications

• Antigen: Rat IgG1 kappa
• Clone: eBRG1
• Conjugate: PE-Cyanine7
• Gene Alias: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Not Applicable
• Form: Liquid

• Applications: Control, Flow Cytometry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG1 κ

For Research Use Only.