Mouse IgG1 kappa, PE-Cyanine7, clone: P188.8.131.52.1, Isotype Control, eBioscience™
Mouse Isotype Control
Brand: Affymetrix eBioscience 25-4714-41
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: PE-Cy7 Mouse IgG1, K Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: This P184.108.40.206.1 antibody is offered in 2 formats: - μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This Cyanine7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
For Research Use Only.