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Mouse IgG1 kappa, PE-Cyanine7, clone: P3.6.2.8.1, Isotype Control, eBioscience™

Mouse Isotype Control

Brand:  Affymetrix eBioscience 25-4714-41

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 14811041

  • £70.66 / 25 tests


EU Stock 17
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Description

Description

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: PE-Cy7 Mouse IgG1, K Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: This P3.6.2.8.1 antibody is offered in 2 formats: - μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgG1 κ
Control
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
IgG1, IgG1k
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This Cyanine7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Monoclonal
Flow Cytometry
P3.6.2.8.1
PE-Cyanine7
Conjugated
Murine
Affinity chromatography
RUO
Isotype Control
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