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F(ab')2-Goat anti-Mouse IgG, eFluor 570, Polyclonal, Secondary Antibody, eBioscience™

F(ab')2-Goat Polyclonal Secondary Antibody

Brand:  Affymetrix eBioscience 41-4010-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15578926

  • £32.05 / 25Āµg


Estimated Shipment date
from Supplier 29-06-2018
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Description

Description

Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).Applications Reported: This polyclonal antibody has been reported for use in immunohistochemical staining, and immunocytochemistry.Applications Tested: This polyclonal F(ab')2 Anti-Mouse IgG antibody has been tested by immunocytochemistry after staining with a primary antibody of the mouse isotype. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

IgG, F(ab')2
Polyclonal
eFluor 570
Conjugated
Goat
Affinity chromatography
RUO
Secondary Antibody
Polyclonal
ImmunocytochemistryĆ¾Immunohistochemistry
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Goat Anti-Mouse IgG
IgG
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Secondary
Murine
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