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Mouse IgD, Functional Grade, clone: 10.4.22, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5924-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15517796

  • £49.41 / 100µg


Estimated Shipment date
from Supplier 28-06-2018
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Description

Description

Description: This 10.4.22 monoclonal antibody reacts with the a allotype of mouse IgD, which is expressed by mature peripheral B cells. This antibody has been reported to activate B cells via engagement of the IgD receptor. The use of anti-IgM as a polyclonal B cell activator is problematic due to interference from high levels of circulating serum IgM.Crossblocking studies suggest that the 10.4.22 antibody recognizes a different epitope from 11-26c.Applications Reported: This 10.4.22 antibody has been reported for use in functional assays.Applications Tested: This 10.4.22 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

IgD
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
Purified
Ighd
IgG2a, kappa
100μg
Store at 2-8°C.
Primary
380797
Functional AssayþIn vitro Assay
10.4.22
Functional Grade
Ighd
Immunoglobulin D, Ig-D
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
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