missing translation for 'onlineSavingsMsg'
Learn More

Human IgD, PerCP-eFluor™ 710, clone: IA6-2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9868-41

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15549456

  • £92.79 / 25 tests


EU Stock 1
Add to basket

Description

Description

Description: This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD). This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.Applications Reported: This IA6-2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This IA6-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgD
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Conjugated
IGHD
IgG2a, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3495
Flow Cytometry
IA6-2
PerCP-eFluor 710
IGHD
Immunoglobulin D
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Videos

For Research Use Only.