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IFN gamma, eFluor 450, clone: XMG1.2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-7311-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341830

  • £165.28 / 100µg


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IFN gamma
XMG1.2
eFluor 450
Conjugated
Rat
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interferon-gamma, IFN-g, IFNg
IgG1
RUO
Primary
Murine
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