IFN gamma, Functional Grade, clone: R4-6A2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7312-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-gamma ), a homodimeric 15-20kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-gamma is an ∽20kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.Applications Reported: The R4-6A2 antibody has been reported for use in cytokine neutralization.Applications Tested: The Functional Grade Purified R4-6A2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested in bioassay for neutralization of IFN gamma bioactivity. The R4-6A2 antibody at 15 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse IFN gamma (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|Store at 2-8°C.|
For Research Use Only.