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IFN gamma, PE-Cyanine7, clone: 4S.B3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-7319-41

Code : Z2

Additional Details : Weight : 0.50000kg

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Product Code. 13417646

  • £78.88 / 25 tests


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.Applications Reported: The 4S.B3 antibody has been reported for use in intracellular flow cytometric analysis.Applications Tested: This 4S.B3 antibody has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL cell sample + 100μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IFN gamma
4S.B3
PE-Cyanine7
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL; 5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interferon gamma
IgG1
RUO
Primary
Human
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