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IFN gamma, FITC, clone: 4S.B3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-7319-81

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15298359

  • £75.06 / 50µg


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.Applications Reported: The 4S.B3 antibody has been reported for use in intracellular staining for flow cytometric analysis.Applications Tested: This 4S.B3 antibody is offered in 2 formats: - μg size: has been tested by intracellular flow cytometric analysis of activated human cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular flow cytometric analysis of activated human cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IFN gamma
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01579
Interferon gamma
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
4S.B3
FITC
IFNG
Conjugated
IFNG
IgG1, kappa
50μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3458
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