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IFN gamma, eFluor 450, clone: 4S.B3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-7319-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15559816

  • £82.61 / 25 tests


Estimated Shipment date
from Supplier 29-06-2018
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Description

Description

Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.Applications Reported: This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 4S.B3 antibody has been pre-titrated and tested by intracellular staining and flwo cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IFN gamma
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P01579
Interferon gamma
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
4S.B3
eFluor 450
IFNG
Conjugated
IFNG
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3458
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