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IDO Monoclonal Antibody (mIDO-48), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£153.00 - £333.00

Specifications

Antigen IDO
Clone mIDO-48
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15835343
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Affymetrix eBioscience
12-9473-80
25 μg
£153.00
25µg
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Estimated Shipment: 15-04-2024
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15845343
View Documents Promotion Details
Affymetrix eBioscience
12-9473-82
100 μg
£333.00
100µg
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Estimated Shipment: 15-04-2024
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Description

Description

Description: The monoclonal antibody mIDO-48 reacts with mouse indoleamine 2,3-dioxygenase 1(IDO1). IDO1 is an enzyme expressed intracellularly by dendritic cells, interferon gamma activated macrophages, epithelial cells, vascular endothelium and tumor cells. This enzyme catalyzes tryptophan into kynurenine metabolites. The reduction of tryptophan and the introduction of kynurenine metabolites in the microenvironment of IDO-expressing cells suppress T cell proliferation and promote the activation and induction of regulatory T cells. Applications Reported: This mIDO-48 antibody has been reported for use in flow cytometric analysis. Applications Tested: This mIDO-48 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product No. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.

IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
Specifications

Specifications

IDO
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
EC 1.13.11.52; IDO; IDO1; IDO-1; INDO; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; indoleamine 2,3-dioxygenase 1; indoleamine 23-dioxygenase; Indoleamine-2; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase
Ido1
Primary
4° C, store in dark, DO NOT FREEZE!
Ido1
mIDO-48
Flow Cytometry
PE
Rat
Mouse
P28776
15930
IgG2b κ
Affinity chromatography
Antibody
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