IDO Mouse anti-Human, eFluor™ 660, Clone: V1NC3IDO, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9750-80

Product Code. 15540117

Description

Description: The monoclonal antibody V1NC3IDO recognizes human IDO. IDO (indoleamine-2,3-dioxygenase, INDO, IDO1) is an intracellular enzyme that catalyzes the degradation of tryptophan to kynurenines. IDO is expressed in a wide variety of tissues and cells, including macrophages, plasmacytoid dendritic cells, and several cell lines. IDO can be induced in many different cell types by IFN gamma or other inflammatory stimuli. Expression of IDO in antigen presenting cells and tumors is thought to mediate immune suppression through depletion of this essential amino acid and/or by the creation of tryptophan metabolites that cause apoptosis of T cells and induction of regulatory T cells. This V1NC3IDO antibody reacts with IDO1 and does not cross react with IDO2. Applications Reported: This V1NC3IDO antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This V1NC3IDO antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. Gamma-interferon (IFNG, MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO, EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.

Specifications

• Antigen: IDO
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P14902
• Gene Symbols: IDO1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3620
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: V1NC3IDO
• Conjugate: eFluor 660
• Gene: IDO1
• Gene Alias: IDO, INDO
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.