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IkB zeta, PerCP-eFluor™ 710, clone: LK2NAP, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-6801-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15371380

  • £84.46 / 25µg


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The LK2NAP monoclonal antibody reacts with mouse IkB zeta (INAP, MAIL). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors and also shares structural homology with Bcl-3. It is induced in monocytes, macrophages and B cells in response to TLR and IL-1R signaling. In NK cells, IkBz is rapidly upregulated in response to IL-12 and IL-18 and is necessary for production of IFN gamma. In T cells, IkBz is not necessary for IFN gamma and IL-4 production by Th1 and Th2 cells, respectively. However in Th17 cells, IkBz acts in concert with RORa and RORgt to upregulate IL-17A, IL-17F, IL-21, IL-22 and IL-23R expression.Applications Reported: This LK2NAP antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This LK2NAP antibody has been tested by intracellular staining and flow cytometric analysis of LPS-activated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

I kappa B zeta
LK2NAP
PerCP-eFluor 710
Conjugated
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
IKBZ, INAP, MAIL, NF-kappa-B inhibitor zeta, Nfkbiz
IgG2a
RUO
Primary
Murine
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