HySystem™ Hydrogels

Provide a compliant, viscoelastic matrix that is physiologically relevant for stem cells

Overview
Brand: GE Healthcare Hyclone

Manufacturer Part Number: SV30140.02

HyStem-HP Hydrogel Kit 7,5mL

UNSPSC: 12352207

Code: 85

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11507310

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Description and Specification

Specification

Format Liquid
Product Type HyStem-HP Hydrogel Kit
Quantity 7.5mL
Solution Type HyStem-HP
Storage Requirements -10°C

HyStem Hydrogel kits are optimal for culturing stem cells whose natural environment is rich in hyaluronic acid (HA). It is xeno-free since its two components are thiol-modified hyaluronan (HyStem) and a thiol-reactive crosslinker, polyethylene glycol diacrylate (Extralink). Extralink is made by adding acrylate groups to both ends of a polyethylene glycol (PEG) polymer. PEG is derived from petroleum and inorganic sources and contains no animal-sourced materials. The hyaluronic acid used to produce HyStem is made by a proprietary bacterial fermentation process which is 100 percent free of animal-derived raw materials. The resultant materials are pure, low in endotoxin, and widely used in a variety of applications. No animalderived ingredients are used in its production.

  • Regulatory friendlysubstrate
  • Lot-to-lot consistency for reliable results
  • Substrate is suitable for both in vitro and in vivo experimentation
  • Fully-defined matrix allows researchers tovcustomize a stem cell niche
  • Allows complete control over matrix characteristics by changing hydrogel stiffness or adding user-supplied extracellular matrix (ECM) proteins and growth factors
  • Tailored HyStem can then be used to coat a plate or encapsulate cells
  • Will gel at room temperature within 30 minutes with no low temperature or low pH steps in its preparatio

HyStem

  • Animal-free: Chemically modified hyaluronan crosslinked with PEGDA
  • Simple to use: Just add water and go
  • Easy to modify: Can change formulation or stiffness before gelation
  • Applications include user-designed surface coatings and differentiation of neurospheres in bioreactors
HyStem-C
  • HyStem but with chemically modified gelatin added
  • Provides an excellent additive to start with when optimizing your own matrix/surface
  • Applications include Culture of Neural progenitors, expansion and differentiation of neurons from neurospheres (depending on the ratio of HyStem to Gelin-S); MSC differentiation
HyStem-HP
  • HyStem-C but with chemically modified heparin added
  • Contains heparin which acts by ionically binding to growth factors for slow delivery of growth factors to cells

Applications:

The following stem cells have been cultured in HyStem: Human embryonic stem cells (H9s); Umbilical cord blood CD34+ stem cells; Hepatic stem cells; Hepatic progenitor cells; Adipose derived stem cells; Mesenchymal stem cells