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Abnova™ Human WNK3 (NP_065973.2, 1 a.a. - 434 a.a.) Partial Recombinant Protein with GST-tag expressed in baculovirus infected Sf21 cells
Used for Func, SDS-PAGE
Brand: Abnova™ P5664.5ug
Additional Details : Weight : 0.02000kg
Description
Members of the 'with no lysine' (WNK) kinase family, such as WNK3, are serine-threonine protein kinases that lack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function (Holden et al., 2004 [PubMed 15194194]).[supplied by OMIM]
Specifications
NP_065973.2 | |
Liquid | |
76kDa | |
Baculovirus infected insect cell (Sf21) expression system | |
Loading 1 ug protein in SDS-PAGE | |
Store at -80°C. Aliquot to avoid repeated freezing and thawing. | |
KIAA1566/PRKWNK3 | |
WNK3 | |
Baculovirus | |
GST | |
Liquid |
Functional Study, SDS-PAGE | |
65267 | |
WNK3 (Human) Recombinant Protein | |
Glutathione sepharose chromatography | |
5 ug | |
RUO | |
WNK3 | |
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: SPAKtide. ATP: 100 uM. | |
Recombinant | |
Baculovirus infected insect cell (Sf21) expression system | |
92% by SDS-PAGE/CBB staining |